Studies of Cellular Metabolism
Glucose Consumption and Lactate Extrusion
3.6.11.
Rates of glucose consumption and lactate extrusion are indicative of the metabolic regimen of a cell culture. and allow the study of effects of chemical compounds on live cells. by comparing the basal rates of an unperturbed culture with rates in the presence of increasing concentrations of drugs. Glucose and lactate sensing was performed through the use of a two-step, NAD-linked enzymatic assay monitored in real time, by absorbance of NADH at 340 nm. The automated microassays have a linear dynamic range for glucose of 0.1-5.6 mM and for lactate 0.05-1.00 mM, while using 40 mL of the enzyme and 3 μL of sample. Method was tested on a murine hepatocyte cell line (TABX2S) adhered to Cytopore beads, which were trapped in a microbioreactor (volume 3 mL /10^5 cells), integrated into an LOV module that comprises a microbioreactor, as shown in 3.6.10.
